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(PDF) Subcellular distribution of GABA receptors in …

[3H]GABA (0.5 pM) was added to the sample and the incubation was continued for 10 min. The samples were then filtered onto a Whatman GF-A glass fiber filter placed in a swinnex filtering unit (Millipore Co.). A cylinder was attached to the top of the filter unit so that one ml of …

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Mutation of the Drosophila vesicular GABA …

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The reverse primer 5′-GCTCTAGACTAAAATGGAAGACCGAT-3′ contains the last 18 nucleotides of the predicted open reading frame and an Xba I restriction site. The location of additional primer pairs used to amplify the 5′ (r1/u1, r1/u2, r1/u3) and 3′ untranslated …

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Mutation of the Drosophila vesicular GABA …

2010-5-1 · The vesicular GABA transporter (VGAT) is required for the storage of GABA in synaptic vesicles and provides a potentially useful probe for inhibitory circuits. However, specific pharmacologic agents for VGAT are not available, and VGAT knockout mice are embryonically lethal, thus precluding behavioral studies.